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Download e-book for kindle: Cell-Based Microarrays: Review of Applications, Developments by Ella Palmer

By Ella Palmer

ISBN-10: 1493905937

ISBN-13: 9781493905935

ISBN-10: 1493905945

ISBN-13: 9781493905942

This publication is a evaluation at the evolution of cell-based microarrays and an replace to the author's past e-book Methods in Molecular Biology: Cell-Based Microarrays. in view that their improvement in 2001, cell-based microarrays have complicated significantly to incorporate expression arrays, brief interfering RNA arrays and antibody arrays. the outside used to coat the glass slides has additionally been considerably greater to permit non-adherent cells to bind to the arrays.

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7] report a storage time of up to 15 months. The second additive, fibronectin, increases the adherence of cells to the spots, reducing their propensity to migrate away once they have been transfected. A further potential function of fibronectin was discussed by [8]: By comparing the cell morphology of human mesenchymal stem cells (hMSCs) in the absence and the presence of fibronectin, they observed that both the cells and the nuclei spread much earlier and to a larger extent when fibronectin was present, in conjunction with a rapid relocation of actin filaments.

More recently, this group published results obtained on antibody microarrays with higher number of probes (147 antibodies) still targeting CD antigens. This new generation of microarrays (named DotScan™ microarrays) allowed the identification of expression profiles specific to discrete leukemia subtypes [45]. , CD4, which is usually highly specific to T lymphocytes). Once again, the authors outline the need to run counter labeling with appropriate antibodies to strengthen the data issued from one subpopulation.

7 Concluding Remarks The chip-based microarrays discussed in this chapter mostly result in a monolayer of cells, of which only those cells that happen to be seeded on a spot take up the genetic information. Thus, the cells are free to leave “their” spot and move around later on. This lack of confinement might be problematic for some sorts of assays, and can be either overcome by blocking the area outside the spots to cell adherence, as shown by Oehmig et al. [32], by a combination of hydrophobic surfaces and shortened time for adherence [11], or by the generation of microwells [17, 18, 47], which puts up a physical barrier around the cells.

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Cell-Based Microarrays: Review of Applications, Developments and Technological Advances by Ella Palmer

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