By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)
Confocal microscopy with laser scanning expertise yields in-vivo photographs of ocular and ocular adnexal surfaces which are so remarkable that they rival histology when it comes to quality.This distinctive atlas and textbook demonstrates basic in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a variety of mobile constructions utilizing cell-density calculations and establishes correlations among novel optical sections of assorted ailments of the ocular floor and scientific findings. in addition, it helps the translation of novel high-magnification optical sections by way of evaluating corneal and conjunctival imprint cytology with in-vivo photos and describes early inflammatory adjustments in corneal grafts, in addition to corneal conjunctivalisation in limbal stem mobile deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). additionally, it instructs the reader approximately diagnostic and healing follow-up innovations and gives a short creation to functions in different fields similar to dentistry and ear, nostril and throat surgery.
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Additional info for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology
This sensitivity is attributable to the large numbers of nerve fibers that pass through the cornea. Furthermore, the corneal nerves exert an influence on the regulation of epithelial integrity and on wound healing . In vivo visualization of these nerve structures is possible by confocal corneal microscopy. The cornea is innervated primarily by sensory fibers arising from the ophthalmic nerve, a terminal division of the trigeminal nerve. 2 mm and 10 mm. The nerve fiber bundles, which enter the anterior and central stroma in the corneal periph- b 1978;96:2085–2088 ).
A Fig. 3 Normal and pathological tear film. a Normal tear film, noncontact examination. 2 Pathological Findings b up time; the surface of the superficial cells is visible in the area of the dry spot b Fig. 1 Superficial Cells (Up to Approximately 50 mm in Diameter) In the case of the most superficial epithelial cells, bright cell borders and a dark cell nucleus and cytoplasm are readily visualized on confo- cal laser scanning microscopy. The cells characteristically display a polygonal – often hexagonal – shape.
A Subepithelial nerve plexus in the cornea (z=51 µm). b Nerve plexus (z=53 µm) Fig. 44 Schematic illustration (a) and threedimensional reconstruction (b–d) of the corneal epithelium with anterior stroma and nerves in a healthy human subject. b Anterior view. c Posterior view. 2 Pathological Findings a Fig. 45 Trephinates freshly obtained in cases of Fuchs corneal dystrophy, stained with calceinAM/ethidium homodimer and evaluated by confocal microscopy. 0, objective b (water-immersion) ¥60, average 32.
Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)